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Oniichan wa Oshimai! Episode 1 English Subbed
Oniichan wa Oshimai!
Onimai: I'm Now Your Sister!, Onii-chan is Done For, お兄ちゃんはおしまい！
Mahiro Oyama was just a normal erotic-game loving dude...until he woke up one morning as a woman! Turns out his mad-scientist little sister, Mihari, tried out one of her new experiments on him...with a disastrous outcome, as far as Mahiro's concerned! But Mihari is as determined to study him as he is determined to go back to his shut-in, game-playing life, and one thing's for sure...life is going to get a whole lot weirder from here on out!
(Source: Kodansha USA)